Journal: Cell Reports Methods

Article Title: Development of a 3-dimensional organotypic model with characteristics of peripheral sensory nerves

doi: 10.1016/j.crmeth.2024.100835

Figure Lengend Snippet:

Article Snippet: The samples were blocked in blocking buffer (PBS containing 0.1% Tween 20 and 3% BSA) for 1 h at room temperature, and then incubated for 24 h at room temperature with the following primary antibodies: mouse anti-NF200 (1:400, Sigma-Aldrich, #N0142, RRID: AB_477257 ), rabbit anti-CGRP (1:200, Sigma-Aldrich, #C8198, RRID: AB_259091 ), Isolectin B4 (Ib4)-FITC conjugate (1:250, Sigma-Aldrich, #L2895), rabbit anti-MBP (1:1000, Abcam, Cambridge, UK, #ab40390, RRID: AB_1141521 ), mouse anti-Caspr, clone K65/35 (1:100, Merck, Darmstadt, Germany, #MABN69, RRID: AB_10806491 ), rabbit anti-Ezrin (1:200, Cell Signaling Technology, Danvers, MA, USA, #3145, RRID: AB_2100309 ), mouse anti-Tubulin β3 (1:500, BioLegend, San Diego, CA, USA, #801201, RRID: AB_2313773 ), rabbit anti-Nav1.8 (1:200, Alomone labs, Jerusalem, Israel, #ASC-016-GP, RRID: AB_2040188 ), guinea pig anti-TRPV1 (1:200, Neuromics, Edina, MN, USA, #GP14100, RRID: AB_1624142 ), rabbit anti-TRPA1 (1:200, Novus Biologicals, Centennial, CO, USA, #NB110-40763, RRID: AB_715124 ), goat anti-TrkA (1:200, R&D systems, Minneapolis, MN, USA, #AF1056, RRID: AB_2283049 ), goat anti-TrkB (1:200, R&D systems, #AF1494, RRID: AB_2155264 ), rabbit anti-Npy2r (1:50, Sigma-Aldrich, #SAB4502029, RRID: AB_10747296 ), rabbit anti-GFAP (1:1000, Abcam, #ab7260, RRID: AB_305808 ), rabbit anti-S100β (1:1000, Abcam, #ab52642, RRID: AB_882426 ), rabbit anti-Iba1 (1:500, Fujifilm Wako Pure Chemical Industries, #019–19741, RRID: AB_839504 ), mouse anti-CD31 (1:500, Cell Signaling Technology, #3528, RRID: AB_2160882 ) or rabbit anti-ATF3 (1:200, Sigma-Aldrich, #HPA001562, RRID: AB_1078233 ).

Techniques: Virus, Recombinant, Software